PowerPoint
Link for Principles of Biology Lecture Feb 20, 2007
Supplemental materials for the
following paper submitted to the Journal of Biological Rhythms June
2004:
Time-Lapse
Analysis of the Circadian Rhythms of Conidiation and Growth Rate in
Neurospora
Van D. Gooch*, Laura Freeman* and
Patricia L. Lakin-ThomasÝ
*Division of
Science and Mathematics, University of Minnesota - Morris, Morris,
Minnesota
56267 USA; ÝDepartment
of Biology, York University, Toronto, Ontario M3J 1P3, Canada
Movie 1: (38.3
MB) (QuickTime
software needed)
Raw movie
data of Neurospora crassa
growing
on a moving race tube using transmitted/underneath red
safety
lighting for imaging such that when conidia are formed the image
becomes
darker. The tube is moved at the same rate as the growth front is
moving so
that the growth front stays in the same fixed location. (Occasionally
there are
glitches in the movement, but data can still be accurately acquired.).
Data for
Figures 1, 2, and 4 and Table 1 of
the paper are derived from this time-lapse video Note that the mm
positions
described in the figures of the paper equal 260 minus 10 X (the cm
position
seen in the video) (e.g. 120 mm and 107 mm in
the paper corresponds to 14 cm and 15.3 cm on the ruler
in the video, respectively). The
cultures are grown in effective constant darkness at 25 degrees C. See
"Materials and Methods" for other conditions.
Movie 2: (8.5
MB)
Neurospora crassa growing on a static race tube using reflected/overhead
red safety lighting for imaging such that when conidia are formed the
image
becomes lighter. No data in the paper directly result from this
time-lapse
video, but many of the principles presented in the paper are more
easily
comprehended by visualizing a static race tube. (Note that the plots
have an
x-axis of distance rather than time as seen in the paper).